Disruption of the ech42 (Endochitinase-Encoding) Gene Affects Biocontrol Activity in Trichoderma harzianum P1
نویسندگان
چکیده
منابع مشابه
Role of the Trichoderma harzianum endochitinase gene, ech42, in mycoparasitism.
The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown under inducing conditions increased ...
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ELAD, Y., 1. CHET, and J. KATAN. 1980. Trichoderma harzianum: A biocontrol agent effective against Sclerotium rolfsii and Rhizocionia solani. Phytopathology 70:119-121. An isolate of Trichoderma harzianum capable of lysing mycelia of The wheat bran preparation of T. harzianum increased growth of bean Sclerotium rolfsii and Rhizoctonia solani was isolated from a soil naturally plants in a noninf...
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Production of extracellular endochitinase could be increased 5-fold in the mycoparasite fungus Trichoderma harzianum by using the cellulase promoter cbh1 of Trichoderma reesei, whereas the total endochitinase activity increased 10-fold. The cbh1 promoter was not expressed on glucose and sucrose in T. harzianum and was induced by sophorose and on cellulase-inducing medium. The endogenous endochi...
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A plate confrontation experiment is commonly used to study the mechanism by which Trichoderma spp. antagonize and parasitize other fungi. Previous work with chitinase gene expression (ech42) during the precontact period of this process in which cellophane and dialysis membranes separated Trichoderma harzianum and its host Rhizoctonia solani resulted in essentially opposite results. Here, we sho...
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Trichoderma harzianum is a filamentous fungus well adapted to different ecological niches. Owing to its ability to antagonize a wide range of plant pathogens, it is used as a biological control agent in agriculture. Selected strains of T. harzianum are also able to increase the tolerance of plants to biotic and abiotic stresses. However, little is known about the regulatory elements of the T. h...
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ژورنال
عنوان ژورنال: Molecular Plant-Microbe Interactions®
سال: 1999
ISSN: 0894-0282,1943-7706
DOI: 10.1094/mpmi.1999.12.5.419